Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis
Identifieur interne : 000388 ( Main/Exploration ); précédent : 000387; suivant : 000389Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis
Auteurs : Najwa Ben Oujji ; Idriss Bakas ; Georges Istamboulié ; Ihya Ait-Ichou ; Elhabib Ait-Addi ; Régis Rouillon ; Thierry NoguerSource :
- Sensors (Basel, Switzerland) [ 1424-8220 ] ; 2012.
Abstract
This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples. The developed devices showed good analytical performances with limits of detection much lower than the maximum residue limit tolerated by international regulations, as well as a good reproducibility and stability.
Url:
DOI: 10.3390/s120607893
PubMed: 22969377
PubMed Central: 3436006
Affiliations:
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Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis</title><author><name sortKey="Ben Oujji, Najwa" sort="Ben Oujji, Najwa" uniqKey="Ben Oujji N" first="Najwa" last="Ben Oujji">Najwa Ben Oujji</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation><affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email> (I.A.-I.);<email>h.aitaddi@esta.ac.ma</email> (E.A.-A.)</nlm:aff></affiliation></author><author><name sortKey="Bakas, Idriss" sort="Bakas, Idriss" uniqKey="Bakas I" first="Idriss" last="Bakas">Idriss Bakas</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation><affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email> (I.A.-I.);<email>h.aitaddi@esta.ac.ma</email> (E.A.-A.)</nlm:aff></affiliation></author><author><name sortKey="Istamboulie, Georges" sort="Istamboulie, Georges" uniqKey="Istamboulie G" first="Georges" last="Istamboulié">Georges Istamboulié</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation></author><author><name sortKey="Ait Ichou, Ihya" sort="Ait Ichou, Ihya" uniqKey="Ait Ichou I" first="Ihya" last="Ait-Ichou">Ihya Ait-Ichou</name><affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email> (I.A.-I.);<email>h.aitaddi@esta.ac.ma</email> (E.A.-A.)</nlm:aff></affiliation></author><author><name sortKey="Ait Addi, Elhabib" sort="Ait Addi, Elhabib" uniqKey="Ait Addi E" first="Elhabib" last="Ait-Addi">Elhabib Ait-Addi</name><affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email> (I.A.-I.);<email>h.aitaddi@esta.ac.ma</email> (E.A.-A.)</nlm:aff></affiliation></author><author><name sortKey="Rouillon, Regis" sort="Rouillon, Regis" uniqKey="Rouillon R" first="Régis" last="Rouillon">Régis Rouillon</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation></author><author><name sortKey="Noguer, Thierry" sort="Noguer, Thierry" uniqKey="Noguer T" first="Thierry" last="Noguer">Thierry Noguer</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22969377</idno><idno type="pmc">3436006</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3436006</idno><idno type="RBID">PMC:3436006</idno><idno type="doi">10.3390/s120607893</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000474</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000474</idno><idno type="wicri:Area/Pmc/Curation">000473</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000473</idno><idno type="wicri:Area/Pmc/Checkpoint">000265</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000265</idno><idno type="wicri:Area/Ncbi/Merge">000227</idno><idno type="wicri:Area/Ncbi/Curation">000227</idno><idno type="wicri:Area/Ncbi/Checkpoint">000227</idno><idno type="wicri:Area/Main/Merge">000390</idno><idno type="wicri:Area/Main/Curation">000388</idno><idno type="wicri:Area/Main/Exploration">000388</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis</title><author><name sortKey="Ben Oujji, Najwa" sort="Ben Oujji, Najwa" uniqKey="Ben Oujji N" first="Najwa" last="Ben Oujji">Najwa Ben Oujji</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation><affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email> (I.A.-I.);<email>h.aitaddi@esta.ac.ma</email> (E.A.-A.)</nlm:aff></affiliation></author><author><name sortKey="Bakas, Idriss" sort="Bakas, Idriss" uniqKey="Bakas I" first="Idriss" last="Bakas">Idriss Bakas</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation><affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email> (I.A.-I.);<email>h.aitaddi@esta.ac.ma</email> (E.A.-A.)</nlm:aff></affiliation></author><author><name sortKey="Istamboulie, Georges" sort="Istamboulie, Georges" uniqKey="Istamboulie G" first="Georges" last="Istamboulié">Georges Istamboulié</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation></author><author><name sortKey="Ait Ichou, Ihya" sort="Ait Ichou, Ihya" uniqKey="Ait Ichou I" first="Ihya" last="Ait-Ichou">Ihya Ait-Ichou</name><affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email> (I.A.-I.);<email>h.aitaddi@esta.ac.ma</email> (E.A.-A.)</nlm:aff></affiliation></author><author><name sortKey="Ait Addi, Elhabib" sort="Ait Addi, Elhabib" uniqKey="Ait Addi E" first="Elhabib" last="Ait-Addi">Elhabib Ait-Addi</name><affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email> (I.A.-I.);<email>h.aitaddi@esta.ac.ma</email> (E.A.-A.)</nlm:aff></affiliation></author><author><name sortKey="Rouillon, Regis" sort="Rouillon, Regis" uniqKey="Rouillon R" first="Régis" last="Rouillon">Régis Rouillon</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation></author><author><name sortKey="Noguer, Thierry" sort="Noguer, Thierry" uniqKey="Noguer T" first="Thierry" last="Noguer">Thierry Noguer</name><affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email> (N.B.O.);<email>idriss_salame@hotmail.com</email> (I.B.);<email>gistamboulie@yahoo.fr</email> (G.I.);<email>rouillon@univ-perp.fr</email> (R.R.)</nlm:aff></affiliation></author></analytic><series><title level="j">Sensors (Basel, Switzerland)</title><idno type="eISSN">1424-8220</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples. The developed devices showed good analytical performances with limits of detection much lower than the maximum residue limit tolerated by international regulations, as well as a good reproducibility and stability.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Kandylis, P" uniqKey="Kandylis P">P. Kandylis</name></author><author><name sortKey="Vekiari, A S" uniqKey="Vekiari A">A.S. Vekiari</name></author><author><name sortKey="Kanellaki, M" uniqKey="Kanellaki M">M. Kanellaki</name></author><author><name sortKey="Grati, K" uniqKey="Grati K">K. Grati</name></author><author><name sortKey="Msallem, M" uniqKey="Msallem M">M. 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Rossi</name></author><author><name sortKey="Quach, A D" uniqKey="Quach A">A.D. Quach</name></author><author><name sortKey="Rosenzweig, Z" uniqKey="Rosenzweig Z">Z. Rosenzweig</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barthelmebs, L" uniqKey="Barthelmebs L">L. Barthelmebs</name></author><author><name sortKey="Hayat, A" uniqKey="Hayat A">A. Hayat</name></author><author><name sortKey="Wis, A" uniqKey="Wis A">A. Wis</name></author><author><name sortKey="Marty, J L" uniqKey="Marty J">J.-L. Marty</name></author><author><name sortKey="Noguer, T" uniqKey="Noguer T">T. Noguer</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Ait Addi, Elhabib" sort="Ait Addi, Elhabib" uniqKey="Ait Addi E" first="Elhabib" last="Ait-Addi">Elhabib Ait-Addi</name><name sortKey="Ait Ichou, Ihya" sort="Ait Ichou, Ihya" uniqKey="Ait Ichou I" first="Ihya" last="Ait-Ichou">Ihya Ait-Ichou</name><name sortKey="Bakas, Idriss" sort="Bakas, Idriss" uniqKey="Bakas I" first="Idriss" last="Bakas">Idriss Bakas</name><name sortKey="Ben Oujji, Najwa" sort="Ben Oujji, Najwa" uniqKey="Ben Oujji N" first="Najwa" last="Ben Oujji">Najwa Ben Oujji</name><name sortKey="Istamboulie, Georges" sort="Istamboulie, Georges" uniqKey="Istamboulie G" first="Georges" last="Istamboulié">Georges Istamboulié</name><name sortKey="Noguer, Thierry" sort="Noguer, Thierry" uniqKey="Noguer T" first="Thierry" last="Noguer">Thierry Noguer</name><name sortKey="Rouillon, Regis" sort="Rouillon, Regis" uniqKey="Rouillon R" first="Régis" last="Rouillon">Régis Rouillon</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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